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西方墨點法為檢測蛋白質方法的一種,透過電泳分離和抗體結合,定量檢體中的蛋白質。可針對相關蛋白進行對於影響細胞生理作用的機制探討。 abcam, WB, Western blot, 西方墨點法, 蛋白, 定量

abcam - Western Blot Tool 你的WB小幫手 :D


abcam 提供一系列的 WB 工具,讓您可順利的完成實驗!

Western blot Protocol:

Sample Preparation and Protein Quantification

Category Product Size AbID
Cell fractionation Cell Fractionation Kit - Standard 1 kit ab109719
Mitochondria/Cytosol Fractionation Kit 25, 100 tests ab65320
Mitochondria Isolation Kit for Cultured Cells 1 kit ab110170
Mitochondria Isolation Kit for Tissue 1 kit ab110168
Plasma Membrane Protein Extraction Kit 50 tests ab65400
Lysosome Isolation Kit 50 tests ab234047
Microsome Isolation Kit 50 tests ab206995
Density gradient media OptiPrep Density Gradient Medium 100, 250 ml ab286850
Lymphoprep 250, 500 ml,


4 packs (4x250 ml)
ab286892
Lysis buffer 10X RIPA Buffer 15 ml ab156034
1X Native Lysis Buffer (Dodecyl maltoside) 15 ml ab156035
10X Cell Lysis Buffer (Triton X-100) 15 ml ab152163
10X Red Blood Cell (RBC) Lysis Buffer 100 ml ab204733
Protease Inhibitor Cocktail Protease Inhibitor Cocktail (EDTA provided in a separate vial) 3, 10, 30 ml ab271306
Protease Inhibitor Cocktail EDTA Free 3, 10, 30 ml ab270055
Phosphatase Inhibitor Cocktail I 1 ml ab201112
Protease and Phosphatase Inhibitor Cocktail (EDTA provided in a separate vial) 1 kit ab201119
Protease and Phosphatase Inhibitor Cocktail (EDTA Free) 1 ml ab201120
Protein quantification BCA Protein Assay Kit II (25 - 2000 μg/ml) 2500, 5000 tests ab287853
BCA Protein Assay Kit (0.5 - 30 μg) 1000 tests ab102536
Bradford Assay Kit 1000 tests ab102535

Protein ladders & WB marker pen

Category Product Size AbID
Prestained Prestained Protein Ladder – Broad molecular weight (10-245 kDa) 500 μl ab116028
Prestained Protein Ladder – Extra broad molecular weight (5-245 kDa) 500 μl ab116029
Prestained Protein Ladder – Extra broad molecular weight (6.5-270 kDa) 500 μl ab234592
Prestained Protein Ladder – Mid-range molecular weight (10-180 kDa) 500 μl ab116027
Unstained Unstained Protein Ladder (10-200 kDa) 500 μl ab234618
WB Marker Pen Western Blot Marker Pen 1 unit ab166858

Transferring & Blocking

Category Product Size AbID
Transfer membrane Low Fluorescence Western Membrane (PVDF) 10 membranes ab133411
Nitrocellulose Transfer Membrane - 0.45 μm 10 membranes ab133412
Nitrocellulose Transfer Membrane - 0.22 μm 10 membranes ab133413
Protein stain Ponceau S Solution 1000 ml ab270042
BlockingInstantBlue® Coomassie Protein Stain (ISB1L) 1000 ml ab119211
Blocking buffer 10X Blocking Buffer 50 ml ab126587

Secondary antibodies

Category Product Size AbID
HRP conjugated

secondary antibodies
Goat Anti-Rabbit IgG H&L (HRP) 1 mg ab6721
Goat Anti-Rabbit IgG H&L (HRP) 500 μg ab205718
Rabbit Anti-Mouse IgG H&L (HRP) 1 mg ab6728
Goat Anti-Mouse IgG H&L (HRP) 1 mg ab6789
Goat Anti-Mouse IgG H&L (HRP) 1 mg ab97023
Goat Anti-Mouse IgG H&L (HRP) 500 μg ab205719
Rabbit Anti-Goat IgG H&L (HRP) 1 mg ab6741

Detection & Stripping

Category Product Size AbID
ECL substrate kit ECL Western Blotting Substrate Kit 50, 500 tests ab65623
ECL Substrate Kit (High Sensitivity) (23 pg - 187 ng) 20, 200, 500 ml ab133406
ECL Substrate Kit (Very High Sensitivity) (4.6 pg - 4.7 ng) 20, 100, 200 ml ab133408
ECL Substrate Kit (Ultra High Sensitivity) (1.2 pg - 2 ng) 20, 100, 200 ml ab133409
Stripping buffer Western Blot Stripping Buffer 500 ml ab282569


更多產品資訊歡迎與 abcam 台灣一級代理—昶安生技 洽詢。


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發佈者 發佈時間 Nov 01, 2023 瀏覽次數 170
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