abcam - Western Blot Tool 你的WB小幫手 :D
abcam 提供一系列的 WB 工具，讓您可順利的完成實驗!
Western blot Protocol:
Sample Preparation and Protein Quantification
| Category | Product | Size | AbID |
| Cell fractionation | Cell Fractionation Kit - Standard | 1 kit | ab109719 |
| Mitochondria/Cytosol Fractionation Kit | 25, 100 tests | ab65320 | |
| Mitochondria Isolation Kit for Cultured Cells | 1 kit | ab110170 | |
| Mitochondria Isolation Kit for Tissue | 1 kit | ab110168 | |
| Plasma Membrane Protein Extraction Kit | 50 tests | ab65400 | |
| Lysosome Isolation Kit | 50 tests | ab234047 | |
| Microsome Isolation Kit | 50 tests | ab206995 | |
| Density gradient media | OptiPrep Density Gradient Medium | 100, 250 ml | ab286850 |
| Lymphoprep | 250, 500 ml, 4 packs (4x250 ml) |
ab286892 | |
| Lysis buffer | 10X RIPA Buffer | 15 ml | ab156034 |
| 1X Native Lysis Buffer (Dodecyl maltoside) | 15 ml | ab156035 | |
| 10X Cell Lysis Buffer (Triton X-100) | 15 ml | ab152163 | |
| 10X Red Blood Cell (RBC) Lysis Buffer | 100 ml | ab204733 | |
| Protease Inhibitor Cocktail | Protease Inhibitor Cocktail (EDTA provided in a separate vial) | 3, 10, 30 ml | ab271306 |
| Protease Inhibitor Cocktail EDTA Free | 3, 10, 30 ml | ab270055 | |
| Phosphatase Inhibitor Cocktail I | 1 ml | ab201112 | |
| Protease and Phosphatase Inhibitor Cocktail (EDTA provided in a separate vial) | 1 kit | ab201119 | |
| Protease and Phosphatase Inhibitor Cocktail (EDTA Free) | 1 ml | ab201120 | |
| Protein quantification | BCA Protein Assay Kit II (25 - 2000 μg/ml) | 2500, 5000 tests | ab287853 |
| BCA Protein Assay Kit (0.5 - 30 μg) | 1000 tests | ab102536 | |
| Bradford Assay Kit | 1000 tests | ab102535 |
Protein ladders & WB marker pen
| Category | Product | Size | AbID |
| Prestained | Prestained Protein Ladder – Broad molecular weight (10-245 kDa) | 500 μl | ab116028 |
| Prestained Protein Ladder – Extra broad molecular weight (5-245 kDa) | 500 μl | ab116029 | |
| Prestained Protein Ladder – Extra broad molecular weight (6.5-270 kDa) | 500 μl | ab234592 | |
| Prestained Protein Ladder – Mid-range molecular weight (10-180 kDa) | 500 μl | ab116027 | |
| Unstained | Unstained Protein Ladder (10-200 kDa) | 500 μl | ab234618 |
| WB Marker Pen | Western Blot Marker Pen | 1 unit | ab166858 |
Transferring & Blocking
| Category | Product | Size | AbID |
| Transfer membrane | Low Fluorescence Western Membrane (PVDF) | 10 membranes | ab133411 |
| Nitrocellulose Transfer Membrane - 0.45 μm | 10 membranes | ab133412 | |
| Nitrocellulose Transfer Membrane - 0.22 μm | 10 membranes | ab133413 | |
| Protein stain | Ponceau S Solution | 1000 ml | ab270042 |
| BlockingInstantBlue® Coomassie Protein Stain (ISB1L) | 1000 ml | ab119211 | |
| Blocking buffer | 10X Blocking Buffer | 50 ml | ab126587 |
Secondary antibodies
| Category | Product | Size | AbID |
| HRP conjugated secondary antibodies |
Goat Anti-Rabbit IgG H&L (HRP) | 1 mg | ab6721 |
| Goat Anti-Rabbit IgG H&L (HRP) | 500 μg | ab205718 | |
| Rabbit Anti-Mouse IgG H&L (HRP) | 1 mg | ab6728 | |
| Goat Anti-Mouse IgG H&L (HRP) | 1 mg | ab6789 | |
| Goat Anti-Mouse IgG H&L (HRP) | 1 mg | ab97023 | |
| Goat Anti-Mouse IgG H&L (HRP) | 500 μg | ab205719 | |
| Rabbit Anti-Goat IgG H&L (HRP) | 1 mg | ab6741 |
Detection & Stripping
| Category | Product | Size | AbID |
| ECL substrate kit | ECL Western Blotting Substrate Kit | 50, 500 tests | ab65623 |
| ECL Substrate Kit (High Sensitivity) (23 pg - 187 ng) | 20, 200, 500 ml | ab133406 | |
| ECL Substrate Kit (Very High Sensitivity) (4.6 pg - 4.7 ng) | 20, 100, 200 ml | ab133408 | |
| ECL Substrate Kit (Ultra High Sensitivity) (1.2 pg - 2 ng) | 20, 100, 200 ml | ab133409 | |
| Stripping buffer | Western Blot Stripping Buffer | 500 ml | ab282569 |
更多產品資訊歡迎與 abcam 台灣一級代理—昶安生技 洽詢。
發佈者
發佈時間 Nov 01, 2023
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