Abcam - 多重組織染色 Multiplex Imaging
多重組織染色 Tools to power multiplex imaging
利用多重組織影像技術來觀察腫瘤組織樣本，有助於發現生物標記，並提高預測患者治療反應的準確性。 Abcam 提供高專一性的重組抗體 (Recombinant antibody)，可對腫瘤微環境 (Tumor microenvironment, TME) 中的關鍵指標進行多重影像分析。
Abcam 廣泛的抗體產品包含了已知和新興的免疫腫瘤學指標，可以有效應用於不同的平台上，並與不同的標定方法結合，提供多元靈活的多重影像分析。
Multiplex IHC 驗證抗體
– 高靈敏度和特異性
– 高再現性
– 經過 TMAs 或 IMC™ 多重染色實驗驗證
– 大部分抗體均經過基因剔除 (Knock-out) 細胞驗證
– 無載體 (Carrier-free) 抗體不含 BSA 和 sodium azide 可用於抗體標定 (Conjugation)
– 可與多種影像設備搭配使用
| Target | Clone name | Species | Reactivity | Applications | Cat no. | KO Validation | Carrier-free |
| 53BP1 | EPR2172(2) | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab222232 | ✔ | ✔ |
| ACADVL/VLCAD | EPR15107(B) | Rabbit | Human | Flow Cyt (Intra), IHC-P, IP, WB | ab231827 | ✔ | ✔ |
| Alpha smooth muscle actin | EPR5368 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab220795 | ✔ | ✔ |
| Alpha smooth muscle actin | 1A4 | Mouse | Human, Mouse, Rat | ICC/IF, IHC-P, WB | ab240654 | ✔ | ✔ |
| Alpha-synuclein | MJFR1 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB | ab209420 | ✔ | ✔ |
| ATG7 | EP1759Y | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab227564 | ✔ | ✔ |
| Bcl-2 | EPR17509 | Rabbit | Human, Mouse | Flow Cyt (Intra), IHC-P, WB | ab219608 | ✔ | ✔ |
| Beta Catenin | E247 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, IP, WB | ab196204 | ✔ | ✔ |
| Beta III Tubulin | EP1569Y | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC | ab221935 | ✔ | ✔ |
| BIN1 | EPR13463 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab250618 | ✔ | ✔ |
| CD3 epsilon | EP449E | Rabbit | Human | IHC-P, IP, WB | ab271850 | ✔ | |
| CD4 | EPR6855 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, Mass Cytometry, WB | ab181724 | ✔ | |
| CD8 alpha | CAL66 | Rabbit | Human, Rat | Flow Cyt, ICC/IF, IHC-P, IP, mIHC | ab251596 | ✔ | |
| CD11b | EPR1344 | Rabbit | Human, Mouse, Rat | IHC-P, WB | ab209970 | ✔ | |
| CD11c | EPR21826 | Rabbit | Mouse | Flow Cyt, IHC-P | ab240558 | ✔ | |
| CD16 | EPR16784 | Rabbit | Human, Rat, Recombinant fragment | IHC-P, Mass Cytometry, WB | ab215977 | low-endotoxin, azide-free | |
| CD20 | SP32 | Rabbit | Human, Mouse, Rat | Flow Cyt (intra), ICC/IF, IHC-P, WB | ab236434 | ✔ | |
| CD27 | EPR8569 | Rabbit | Human | Flow Cyt, ICC/IF, IHC-P, WB | ab256583 | ✔ | ✔ |
| CD31 | EP1601Y | Rabbit | Human | Flow Cyt (Intra), IHC-P, WB | ab226157 | ✔ | |
| CD39 | EPR20627 | Rabbit | Human, Mouse | IHC-P, Mass Cytometry, WB | ab236038 | ✔ | |
| CD40 | EPR20540 | Rabbit | Human | IHC-P, Mass Cytometry, WB | ab223546 | ✔ | ✔ |
| CD45 | EP322Y | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab214437 | ✔ | |
| CD68 | EPR20545 | Rabbit | Human | ICC/IF, IHC-P, WB, mIHC | ab227458 | ✔ | |
| CD68 | SP251 | Rabbit | Human | Flow Cyt, IHC-P, mIHC | ab271959 | ✔ | |
| CD74 | EPR4064 | Rabbit | Human | Flow Cyt (Intra), I-ELISA, ICC/IF, IHC-P, WB | ab247655 | ✔ | ✔ |
| CD161 | EPR26340-6 | Rabbit | Human | Flow Cyt, ICC/IF, IHC-P, mIHC | ab302565 | ✔ | |
| CD163 | EPR19518 | Rabbit | Human, Mouse, Rat | Flow Cyt, IHC-Fr, IHC-P, WB | ab213612 | ✔ | |
| Chromogranin A | EPR22537-249 | Rabbit | Human, Mouse, Rat | IHC-Fr, IHC-P, WB, mIHC | ab256169 | ✔ | |
| Claudin 5 | EPR7583 | Rabbit | Human, Mouse | ICC/IF, IHC-P, Mass Cytometry, WB | ab236066 | ✔ | |
| Collagen VI | EPR17072 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, WB, mIHC | ab271938 | ✔ | ✔ |
| CPT2 | EPR13626 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, WB | ab231162 | ✔ | ✔ |
| CXCR4 | EPUMBR3 | Rabbit | Human, Mouse | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab271934 | ✔ | ✔ |
| Cytokeratin 7 | EPR1619Y | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab181831 | ✔ | ✔ |
| Cytokeratin 13 | EPR3671 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab239918 | ✔ | ✔ |
| EGFR | EP38Y | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), I-ELISA, ICC/IF, IHC-P, IP, WB | ab272293 | ✔ | ✔ |
| EpCAM | EPR20532-225 | Rabbit | Human | Flow Cyt, ICC/IF, IHC-P, IP, WB, mIHC | ab225894 | ✔ | ✔ |
| Fibronectin | EPR23110-46 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-Fr, IHC-P, WB | ab268022 | ✔ | ✔ |
| FOXP3 | 236A/E7 | Mouse | Human | IHC-P, WB, mIHC | ab96048 | ✔ | |
| Gastric Mucin/MUC-6 | EPR20623 | Rabbit | Human | IHC-P, mIHC | ab251578 | ✔ | |
| GFAP | EPR1034Y | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP, WB, mIHC | ab218309 | ✔ | |
| GLUT1 | EPR3915 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab252403 | ✔ | ✔ |
| Glutathione Synthetase | EPR6563 | Rabbit | Human | Flow Cyt (Intra), IHC-P, WB | ab236062 | ✔ | ✔ |
| Granzyme B | EPR20129-217 | Rabbit | Human | IHC-P, WB, mIHC | ab219803 | ✔ | |
| HADHA | EPR17940 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab231169 | ✔ | ✔ |
| Hexokinase I | EPR10134(B) | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab233837 | ✔ | ✔ |
| Hexokinase II | EPR20839 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab228819 | ✔ | ✔ |
| HLA A | EP1395Y | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab216653 | ✔ | ✔ |
| HLA-DR | EPR3692 | Rabbit | Human, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab209968 | ✔ | |
| IDH1 | EPR21002 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), IHC-P, IP, WB | ab242078 | ✔ | ✔ |
| IDH2 | EPR7577 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab230796 | ✔ | ✔ |
| Indoleamine 2, 3-dioxygenase | EPR20374 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab271990 | ✔ | ✔ |
| IRF3 | EPR2418Y | Rabbit | Human, Mouse | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab201809 | ✔ | ✔ |
| Ki67 | SP6 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC | ab197547 | ✔ | ✔ |
| Ki67 | B56 | Mouse | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P | ab279657 | ✔ | ✔ |
| Lamin B1 | EPR8985(B) | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, IP, WB | ab220797 | ✔ | ✔ |
| Lamin B1 | EPR22165-121 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB, mIHC | ab239399 | ✔ | ✔ |
| Liver FABP | EPR20464 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, WB, mIHC | ab240401 | ✔ | |
| Lysozyme | SP350 | Rabbit | Human | Flow Cyt (Intra), IHC-P, WB, mIHC | ab242430 | ✔ | ✔ |
| MAP2 | EPR22641-106 | Rabbit | Human | IHC-P, mIHC | ab256511 | ✔ | |
| MSH2 | EPR21017-123 | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab228334 | ✔ | |
| MUC2 | EPR23479-47 | Rabbit | Human, Mouse, Rat | IHC-Fr, IHC-P, WB, mIHC | ab272706 | ✔ | |
| Mucin 5AC | 45M1 | Mouse | Human, Mouse, Rat | ICC/IF, IHC-Fr, IHC-P, mIHC | ab212636 | ✔ | |
| MyD88 | EPR590(N) | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab199247 | ✔ | ✔ |
| Myelin PLP | EPR2350-106 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-Fr, IHC-P, IP, WB, mIHC | ab275751 | ✔ | |
| NeuN | EPR12763 | Rabbit | Cat, Common marmoset, Dog, Human, Mouse, Rat, Sheep, Zebrafish | Flow Cyt (Intra), ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB, mIHC | ab209898 | ✔ | |
| NF-kB p65 | E379 | Rabbit | Human, Mouse | ICC/IF, IHC-P, IP, WB | ab207297 | ✔ | ✔ |
| NKG2D | EPR24072-342 | Rabbit | Human | IHC-P, mIHC | ab302908 | ✔ | |
| Olig2 | EPR2673 | Rabbit | Human, Mouse, Rat | ICC/IF, IHC-P, Mass Cytometry, WB | ab220796 | ✔ | |
| P2Y12 | EPR23511-72 | Rabbit | Human | IHC-P, mIHC | ab274386 | ✔ | |
| pan Cytokeratin | C-11 | Mouse | Human, Mouse, Rat | ICC/IF, IHC-Fr, IHC-P, mIHC | ab264485 | ✔ | |
| Pancreatic polypeptide | EPR22853-61 | Rabbit | Human | IHC-P, WB, mIHC | ab256592 | ✔ | |
| PARP1 | EPR18461 | Rabbit | Human, Mouse | ICC/IF, IHC-P, WB | ab222234 | ✔ | ✔ |
| PD1 | CAL20 | Rabbit | Human | ICC/IF, IHC-P, WB, mIHC | ab251613 | ✔ | |
| PDGFR alpha | Y92 | Rabbit | Human, Mouse | ELISA, Flow Cyt (Intra), ICC/IF, IHC-FoFr, IHC-P, IP, WB | ab271835 | ✔ | ✔ |
| PD-L1 | 8月28日 | Rabbit | Human | Flow Cyt, ICC/IF, IHC-P, WB | ab228413 | ✔ | ✔ |
| PD-L1 | CAL10 | Rabbit | Human, Recombinant fragment | ELISA, ICC/IF, IHC-P, IP, WB, mIHC | ab251611 | ✔ | ✔ |
| PKC delta | EPR17075 | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab222229 | ✔ | ✔ |
| Rab3D | EPR8106 | Rabbit | Human | IHC-P, WB, mIHC | ab248251 | ✔ | |
| Rad51 | EPR4030(3) | Rabbit | Human | Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB | ab221796 | ✔ | |
| SHP1 | Y476 | Rabbit | Human, Mouse, Rat | IHC-P, WB | ab226008 | ||
| SOX2 | EPR3131 | Rabbit | Human, Leucoraja erinacea, Mouse, Rat | ICC/IF, IHC-Wmt, IHC-P, Mass Cytometry, WB, sELISA | ab215970 | ✔ | |
| STAT3 | EPR787Y | Rabbit | Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab171359 | ✔ | ✔ |
| Synaptophysin | YE269 | Rabbit | Donkey, Human, Mouse, Rat | ICC/IF, IHC-Fr, IHC-P, WB | ab187259 | ✔ | ✔ |
| Villin | SP145 | Rabbit | Human | Flow Cyt (Intra), IHC-P, IP, WB, mIHC | ab245749 | ✔ | |
| Vimentin | EPR3776 | Rabbit | African green monkey, Human, Mouse, Rat | Flow Cyt (Intra), ICC/IF, IHC-P, WB | ab193555 | ✔ | ✔ |
| YKL-40/CHI3L1 | EPR19078-157 | Rabbit | Human | Flow Cyt (Intra), IHC-P, WB | ab255864 | ✔ | ✔ |
| Stain cycle | Biomarkers | Fluorophore | Antibody concentration | Cat no. | Clone |
| Cycle-1 | aSMA | Alexa Fluor 488 | 1/200 | ab184675 | 1A4 |
| Popoplanin | Alexa Fluor 555 | 1/50 | D2-40 | ||
| CD31 | Alexa Fluor 647 | 1/100 | ab218582 | EPR3094 | |
| Cycle-2 | Cytokeratin 5 | Alexa Fluor 488 | 1/200 | ab193894 | EP1601Y |
| HER2 | Alexa Fluor 555 | 1/50 | 3B5 | ||
| CD44 | Alexa Fluor 647 | 1/200 | ab194988 | EPR1013Y | |
| Cytokeratin 19 | Alexa Fluor 750 | 1/200 | A53-B/A2 | ||
| Cycle-3 | Phospho-Histone H3 | Alexa Fluor 488 | 1/100 | D2C8 | |
| Cytokeratin 14 | Alexa Fluor 555 | 1/400 | ab7800 | LL002 | |
| Ki67 | Alexa Fluor 647 | 1/400 | D3B5 | ||
| CD45 | Alexa Fluor 750 | 1/100 | ab214437 | EP322 |
下載完整 Multiplex Imaging application note
更多產品資訊歡迎與 Abcam 台灣一級代理—昶安生技 洽詢。
發佈者
發佈時間 May 01, 2024
瀏覽次數 94