Apotracker™ Green 細胞凋亡染劑
Apotracker™ Green
(RUO)
Cat.no | Size |
427401 | 20 test |
427402 | 100 test |
427403 | 200 test |
描述 |
Apotracker Green(也稱為 Apo-15 peptide)為細胞凋亡染劑與 Annexin V 為類似產品,但不受鈣離子影響,可用於檢測正在凋亡細胞的磷脂醯絲胺酸 (phosphatidylserine, PS) 殘基外翻到細胞表面時的表現, 此探針可與死細胞指示劑 (Zombie live/dead probes) 結合使用。 |
數據 |
(1) Apotracker™ Green 的信號可以在固定後保留 Day-old murine splenocytes were stained with Apotracker™ Green, washed and then either analyzed unfixed (black) or fixed with FluoroFix™ Buffer (red). Signal could be affected by other harsher fix and permeabilization reagents (2) Apotracker™ Green 可經由流式細胞術及顯微鏡去辨別細胞凋亡 (Left) Day-old splenocytes, gated on Helix NP™ NIR negative cells to exclude necrotic cells. (Right) Helix NP™ NIR-negative cells (red gate, left plot) stained for Apotracker™ Green and BV421™ Annexin V. Staining was performed with Annexin Binding Buffer according to BioLegend protocol. It has not been determined if Annexin V and Apotracker™ Green compete for binding sites. 5-day old HeLa cells were stained with Calcein Red-AM (an indicator of live, healthy cells), Helix NP™ Blue, and Apotracker™ Green (Apo-15). (3) 使用 Apotracker™ Green 標記凋亡細胞,無需使用特定的緩衝液 Unstimulated (left) and CD95-stimulated (right) Jurkat cells were stained with Apotracker™ Green (Apo-15) and Helix NP™ NIR. Cells were stained for 10-15 min in FACS buffer followed by two washes prior to analysis. |
物種反應性 |
Human, Mouse, Rat, All Species |
保存方式 |
保存在 -20 °C 。 |
產品應用 |
流式細胞術/活細胞生物影像 |
步驟說明 |
可添加以下體積,將試劑重新配製為 80 µM, 做為原始濃度館:
(1) 使用 cell staining buffer (cat.no 420201),將 Apotracker 溶液進行 1:10 的稀釋,做為染色的工作濃度。 (2) 並添加 5 μL 工作濃度的 Apotracker 溶液到 100 μL 的 1x10^6 細胞中,可以獲得 400nM 的染色濃度條件,此濃度可針對不同細胞類型進行優化。相對於懸浮細胞中,BioLegend 建議可使用 200-800nM 的濃度範圍。 (3) 在室溫下培養 10-20 分鐘。 (4) 可以搭配細胞死活染劑 (cat.no 423113) ,進行雙重染色。 (5) 清洗兩次細胞後,即可進行流式細胞儀的上機,並適用於 FITC channeal 。 |