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細胞凋亡染劑與 Annexin V 為類似產品,但不受鈣離子影響。 Apotracker™ Green , Annexin V, 細胞凋亡

Apotracker™ Green 細胞凋亡染劑

Apotracker™ Green 

(RUO)

Cat.no

Size

42740120 test
427402

100 test

427403200 test

描述

Apotracker Green(也稱為 Apo-15 peptide)為細胞凋亡染劑與 Annexin V 為類似產品,但不受鈣離子影響,可用於檢測正在凋亡細胞的磷脂醯絲胺酸 (phosphatidylserine, PS) 殘基外翻到細胞表面時的表現, 此探針可與死細胞指示劑 (Zombie live/dead probes) 結合使用。
數據

(1) Apotracker™ Green 的信號可以在固定後保留


Day-old murine splenocytes were stained with Apotracker™ Green, washed and then either analyzed unfixed (black) or fixed with FluoroFix™ Buffer (red). Signal could be affected by other harsher fix and permeabilization reagents


(2) Apotracker™ Green 可經由流式細胞術及顯微鏡去辨別細胞凋亡


(Left) Day-old splenocytes, gated on Helix NP™ NIR negative cells to exclude necrotic cells. (Right) Helix NP™ NIR-negative cells (red gate, left plot) stained for Apotracker™ Green and BV421™ Annexin V. Staining was performed with Annexin Binding Buffer according to BioLegend protocol. It has not been determined if Annexin V and Apotracker™ Green compete for binding sites.


5-day old HeLa cells were stained with Calcein Red-AM (an indicator of live, healthy cells), Helix NP™ Blue, and Apotracker™ Green (Apo-15).


(3) 使用 Apotracker™ Green 標記凋亡細胞,無需使用特定的緩衝液

Unstimulated (left) and CD95-stimulated (right) Jurkat cells were stained with Apotracker™ Green (Apo-15) and Helix NP™ NIR. Cells were stained for 10-15 min in FACS buffer followed by two washes prior to analysis.

物種反應性
Human, Mouse, Rat, All Species
保存方式
保存在 -20 °C 。
產品應用
流式細胞術/活細胞生物影像
步驟說明
可添加以下體積,將試劑重新配製為 80 µM, 做為原始濃度館:
  • 200 test: 添加 200 µL DMSO 
  • 100 test: 添加 100 µL DMSO 
  • 20 test: 添加 20 µL DMSO 

(1) 使用 cell staining buffer (cat.no 420201),將 Apotracker 溶液進行 1:10 的稀釋,做為染色的工作濃度。

(2) 並添加 5 μL 工作濃度的 Apotracker 溶液到 100 μL 的 1x10^6 細胞中,可以獲得 400nM 的染色濃度條件,此濃度可針對不同細胞類型進行優化。相對於懸浮細胞中,BioLegend 建議可使用 200-800nM 的濃度範圍。

(3) 在室溫下培養 10-20 分鐘。

(4) 可以搭配細胞死活染劑 (cat.no 423113) ,進行雙重染色。

(5) 清洗兩次細胞後,即可進行流式細胞儀的上機,並適用於 FITC channeal 。

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發佈者 發佈時間 Sep 15, 2021 瀏覽次數 87
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