BioLegend TotalSeq™ Universal Cocktail 抗體混合物，實現一次性觀察上百種蛋白表現的可能
BioLegend TotalSeq™ Universal Cocktail 抗體混合物，實現一次性觀察上百種蛋白表現的可能
近年來高通量檢測技術蓬勃發展，2023年免疫大會上更看到 CITE-seq 分析技術已成為必備分析項目！
CITE-seq 技術可以在單細胞水平上，通過測序的方法將基因表達信息與細胞表面蛋白信息聯合起來，達到同時檢測細胞表面的蛋白質信息與細胞內轉錄組信息的目的。
這項技術相較於只有轉錄組測序而言，額外地加入了細胞表面蛋白的表達的資訊，聯同細胞內的轉錄組信息，可以更加深入地區分細胞異質性，更精確地挖掘特異性的細胞類型，探索治療耐藥性等生物學現象背後的機制。
使用 TotalSeq™ Antibody Cocktails 實 現一次性觀察上百種蛋白表現的可能 |
BioLegend 為增加實驗的效率，推出 BioLegend TotalSeq™ Antibody Cocktails 抗體混合物，適用於各大單細胞分析平台，簡化單細胞多組學實驗流程，將免疫細胞特性進行前所未有的深度分析！
觀看影片了解 TotalSeq™ Antibody Cocktails
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TotalSeq™ Antibody Cocktails 優點
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單管包裝 | 預滴定找出 | 減少繁瑣的 | 降低單支抗體高成本， | 降低外在因素 |
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TotalSeq™ Antibody 分類選擇
 | TotalSeq-A | - 與使用 poly(dT) oligonucleotides 單細胞分析系統兼容 - Human cocktail 含有 154支抗體 + 9支 isotype control - Mouse cocktail 含有 119支抗體 + 9支 isotype control | Human: 399907 Mouse: 199901 |
 | TotalSeq-B | - 與 10x Genomics Chromium Single Cell Gene Expression Kits with Feature Barcode technology (dual index) 系統兼容 - Human cocktail 含有 134支抗體 + 6支 isotype control - Mouse cocktail 含有 97支抗體 + 7支 isotype control | Human: 399904 Mouse: 199902 |
 | TotalSeq-C | - 與 10x Genomics Single Cell Immune Profiling Solution系統兼容 - Human cocktail 含有 130支抗體 + 7支 isotype control - Mouse cocktail 含有 113支抗體 + 9支 isotype control | Human: 399905 Mouse: 199903 |
Human PBMCs were stained with the TotalSeq-C Human Universal Cocktail v1.0 and processed using the 10x Genomics Single Cell Immune Profiling Solution (5’) and Illumina sequencing. Protein and RNA count data were transformed and visualized in a UMAP projection overlaid with protein and RNA expression levels. Clusters were identified based on protein expression only.
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Resting mouse splenocytes were stained with the TotalSeq-B Mouse Universal Cocktail v1.0 and processed using the 10x Genomics Single Cell 3’ v3.1 featuring barcode kit and Illumina sequencing. Protein and RNA count data were transformed and visualized in a UMAP projection overlaid with protein and RNA expression levels. Clusters were identified based on protein expression only.
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TotalSeq™ TBNK Panel
使用 CD3、CD4、CD8、CD11c、CD14、CD16、CD19、CD45、CD56 在細胞上的表現區分不同的免疫細胞亞型。
※每種抗體使用人類 PBMC優化條件，弱使用裂解的全血或添加其他TotalSeq™抗體則實驗條件需另優化
Human PBMCs were stained with the TotalSeq-B Human TBNK Cocktail and processed using the 10x Genomics Single Cell 3’ v3 feature barcoding kit and Illumina sequencing. Protein count data were transformed and visualized in a UMAP projection overlaid with protein expression levels for each component of the cocktail. Clusters were identified based on protein expression only.
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產品資訊
TotalSeq™-A Human TBNK Cocktail | 399901 |
TotalSeq™-B Human TBNK Cocktail | 399902 |
TotalSeq™-C Human TBNK Cocktail | 399903 |
BioLegend 昶安獨家代理
觀看影片了解 TotalSeq™ Antibody Cocktails 操作流程
BioLegend 昶安獨家代理
更多產品資訊，歡迎洽詢 BioLegend 台灣獨家代理 - 昶安科技。